THE PELOTA LOCUS ENCODES A PROTEIN REQUIRED FOR MEIOTIC CELL-DIVISION - AN ANALYSIS OF G(2)/M ARREST IN DROSOPHILA SPERMATOGENESIS

被引:0
作者
EBERHART, CG [1 ]
WASSERMAN, SA [1 ]
机构
[1] UNIV TEXAS, SW MED CTR, DEPT BIOCHEM, DALLAS, TX 75235 USA
来源
DEVELOPMENT | 1995年 / 121卷 / 10期
关键词
CELL CYCLE; MEIOSIS; CDC25; CHROMOSOME CONDENSATION; DROSOPHILA; PELOTA; SPERMATOGENESIS;
D O I
暂无
中图分类号
Q [生物科学];
学科分类号
07 ; 0710 ; 09 ;
摘要
During Drosophila spermatogenesis, germ cells undergo four rounds of mitosis, an extended premeiotic G(2) phase and two meiotic divisions, In males homozygous for mutations in pelota, the germline mitotic divisions are normal, but the cell cycle arrests prior to the first meiotic division; pelota males are therefore sterile, Chromosomes begin to condense in these mutants, but other meiotic processes, including nuclear envelope breakdown and spindle formation, do not occur, The arrest phenotype closely resembles that of mutations in the Drosophila cdc25 homolog twine. Although meiosis is blocked in pelota and twine homozygotes, spermatid differentiation continues, pelota is also required for patterning in the eye and mitotic divisions in the ovary, We have cloned the pelota locus and show it encodes a 44x10(3) M(r) protein with yeast, plant, worm and human homologs.
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页码:3477 / 3486
页数:10
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