PHYLOGENETIC ANALYSIS OF 2 SYNTROPHIC PROPIONATE-OXIDIZING BACTERIA IN ENRICHMENTS CULTURES

被引：52
作者：
HARMSEN, HJM
KENGEN, KMP
AKKERMANS, ADL
STAMS, AJM
机构：
[1] Department of Microbiology Wageningen, Agricultural University, Wageningen, 6703 CT
来源：
SYSTEMATIC AND APPLIED MICROBIOLOGY | 1995年 / 18卷 / 01期
关键词：
SYNTROPHIC BACTERIA; PROPIONATE DEGRADATION; SULFATE-REDUCING BACTERIA; PCR; 16S RIBOSOMAL-RNA; PHYLOGENY;
D O I：
10.1016/S0723-2020(11)80450-9
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
A 16S rDNA sequence analysis of two syntrophic propionate-oxidizing bacteria was done using PCR amplification of the 16S rRNA-genes from DNA isolated from two enrichment cultures. The two mesophilic propionate-oxidizing bacteria MPOB and KOPROP1 are closely related to Syntrophobacter wolinii and form one cluster within the sulfate-reducing bacteria. Specific oligonucleotides were developed and used in dot blot and in situ hybridization studies. These experiments showed that the obtained sequences were derived from the dominant propionate-oxidizing bacteria in the enrichment cultures.
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页码：67 / 73
页数：7
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