CORRELATION OF BIOCATALYTIC ACTIVITY IN AN ORGANIC AQUEOUS 2-LIQUID PHASE SYSTEM WITH SOLVENT CONCENTRATION IN THE CELL-MEMBRANE

被引：115

作者：

OSBORNE, SJ ^{[1
]}

LEAVER, J ^{[1
]}

TURNER, MK ^{[1
]}

DUNNILL, P ^{[1
]}

机构：

[1] UNIV LONDON UNIV COLL,SERC CTR BIOCHEM ENGN,DEPT CHEM & BIOCHEM ENGN,TORRINGTON PL,LONDON WC1E 7JE,ENGLAND

来源：

ENZYME AND MICROBIAL TECHNOLOGY | 1990年 / 12卷 / 04期

关键词：

membranes; progesterone; 11α-hydroxylase; Rhizopus nigricans; solvent damage; Two-liquid phase biocatalysis;

D O I：

10.1016/0141-0229(90)90100-5

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Results presented here show that loss of progesterone 11α-hydroxylase activity in Rhizopus nigricans in aqueous-organic two-liquid phase and cosolvent systems correlates well with the concentration of solvent in the cell membranes. Rhizopus nigricans is shown to retain full 11α-hydroxylase activity at saturating aqueous phase concentrations of hexane and the higher primary alcohols. This reflects their inability to attain a critical concentration in the cell membranes. The relationship between our own findings and the previously described correlation of the logarithm of the partition coefficient with activity retention is explained and design parameters are proposed that may be used to select solvents for future biocatalytic systems. © 1990.

引用

页码：281 / 291

页数：11

共 33 条

[1] PHASE TOXICITY IN MULTIPHASE BIOCATALYSIS [J].