CONSTRUCTION AND CHARACTERIZATION OF AN ABSOLUTE DELETION MUTANT OF ESCHERICHIA-COLI RIBONUCLEASE-II

被引：0
作者：
PIEDADE, J [1 ]
ZILHAO, R [1 ]
ARRAIANO, CM [1 ]
机构：
[1] UNIV NOVA LISBOA,INST TECNOL QUIM & BIOL,P-2780 OEIRAS,PORTUGAL
来源：
FEMS MICROBIOLOGY LETTERS | 1995年 / 127卷 / 03期
关键词：
ESCHERICHIA COLI; MESSENGER-RNA DEGRADATION; RNB; RIBONUCLEASE II; DELETION;
D O I：
暂无
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
The degradation of mRNA plays a central role in the control of protein synthesis. In Escherichia coli, the mb gene encodes ribonuclease II (RNase II), one of the two main exonucleases involved in mRNA decay. We have constructed strain CMA201, in which the rnb promoter region and the gene were deleted from the chromosome and replaced by a tet(r) cassette, This is the first rnb absolute deletion mutant that shows the complete absence of rnb-specific mRNA. This strain has growth characteristics similar to the wild-type, even though it has no RNase II activity, and it should be useful in studies of mRNA metabolism.
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页码：187 / 193
页数：7
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