PARTIAL-PURIFICATION AND CHARACTERIZATION OF ARYLAMINE N-ACETYLTRANSFERASE IN BOVINE RETINA

被引：7
|
作者：
GAUDET, SJ
CHADER, GJ
机构：
[1] Laboratory of Retinal Cell and Molecular Biology, National Eye Institute, National Institutes of Health, Bethesda, MD
来源：
CURRENT EYE RESEARCH | 1992年 / 11卷 / 12期
关键词：
D O I：
10.3109/02713689208999543
中图分类号：
R77 [眼科学];
学科分类号：
100212 ;
摘要：
Arylamine N-acetyltransferase (NAT) activity was partially purified and characterized in bovine retina. Upon examining the retinal supernatant for multiple ionic species, only one NAT activity was detected. Based upon its substrate specificity, it is best described as an arylamine NAT. According to size-exclusion HPLC, the molecular mass of the arylamine NAT is approximately 30-kDa. This arylamine NAT acetylates p-aminobenzoic acid thereby demonstrating a monomorphic pattern of acetylation. The NAT activity demonstrated low sensitivity to methotrexate inhibition as indicated by a high IC50 value (480 muM).
引用
收藏
页码：1185 / 1192
页数：8
相关论文
共 50 条
  • [21] ARYLAMINE N-ACETYLTRANSFERASE AND ARYLALKYLAMINE N-ACETYLTRANSFERASE IN THE MAMMALIAN PINEAL-GLAND
    VOISIN, P
    NAMBOODIRI, MAA
    KLEIN, DC
    JOURNAL OF BIOLOGICAL CHEMISTRY, 1984, 259 (17) : 913 - 918
  • [22] N-ACYLATION OF TYRAMINES - PURIFICATION AND CHARACTERIZATION OF AN ARYLAMINE N-ACETYLTRANSFERASE FROM RAT-BRAIN AND LIVER
    YU, PH
    BOULTON, AA
    CANADIAN JOURNAL OF BIOCHEMISTRY, 1979, 57 (10): : 1204 - 1209
  • [23] HAMSTER MONOMORPHIC ARYLAMINE N-ACETYLTRANSFERASE - EXPRESSION IN ESCHERICHIA-COLI AND PURIFICATION
    BERGSTROM, CP
    WAGNER, CR
    ANN, DK
    HANNA, PE
    PROTEIN EXPRESSION AND PURIFICATION, 1995, 6 (01) : 45 - 55
  • [24] Insight into cofactor recognition in arylamine N-acetyltransferase enzymes: structure of Mesorhizobium loti arylamine N-acetyltransferase in complex with coenzyme A
    Xu, Ximing
    de la Sierra-Gallay, Ines Li
    Kubiak, Xavier
    Duval, Romain
    Chaffotte, Alain F.
    Dupret, Jean-Marie
    Haouz, Ahmed
    Rodrigues-Lima, Fernando
    ACTA CRYSTALLOGRAPHICA SECTION D-STRUCTURAL BIOLOGY, 2015, 71 : 266 - 273
  • [25] The Characteristics of Arylamine N-Acetyltransferase in Pseudomonas aeruginosa
    Sue-Er Hsieh
    Hsueh-Hsia Lo
    Jing-Gung Chung
    Current Microbiology, 1998, 36 : 353 - 360
  • [26] Arylamine N-acetyltransferase aggregation and constitutive ubiquitylation
    Liu, Fen
    Zhang, Naixia
    Zhou, Xin
    Hanna, Patrick E.
    Wagner, Carston R.
    Koepp, Deanna M.
    Walters, Kylie J.
    JOURNAL OF MOLECULAR BIOLOGY, 2006, 361 (03) : 482 - 492
  • [27] ARYLAMINE N-ACETYLTRANSFERASE ACTIVITY OF THE HUMAN PLACENTA
    DEREWLANY, LO
    KNIE, B
    KOREN, G
    JOURNAL OF PHARMACOLOGY AND EXPERIMENTAL THERAPEUTICS, 1994, 269 (02) : 756 - 760
  • [28] Expression of arylamine N-acetyltransferase in human intestine
    Hickman, D
    Pope, J
    Patil, SD
    Fakis, G
    Smelt, V
    Stanley, LA
    Payton, M
    Unadkat, JD
    Sim, E
    GUT, 1998, 42 (03) : 402 - 409
  • [29] Arylamine N-acetyltransferase in adult mouse brain
    Mo, M
    Wakefield, L
    French, S
    Kawamura, A
    Sim, E
    DRUG METABOLISM REVIEWS, 2004, 36 : 164 - 164
  • [30] Identification and Characterization of Novel Arylamine N-Acetyltransferase Small Molecule Inhibitors
    Leggett, Carmine S.
    Doll, Mark A.
    States, J. Christopher
    Trent, John O.
    Hein, David W.
    FASEB JOURNAL, 2012, 26
12345