LINKAGE MAPPING OF D21S171 TO THE DISTAL LONG ARM OF HUMAN CHROMOSOME-21 USING A POLYMORPHIC (AC)N DINUCLEOTIDE REPEAT

被引：0
作者：
PETERSEN, MB
WEBER, JL
SLAUGENHAUPT, SA
KWITEK, AE
MCINNIS, MG
CHAKRAVARTI, A
ANTONARAKIS, SE
机构：
[1] JOHNS HOPKINS UNIV,SCH MED,CTR MED GENET,DEPT PEDIAT,CMSC 10-111,600 N WOLFE ST,BALTIMORE,MD 21205
[2] UNIV PITTSBURGH,DEPT PSYCHIAT,PITTSBURGH,PA 15261
[3] UNIV PITTSBURGH,DEPT HUMAN GENET,PITTSBURGH,PA 15261
[4] MARSHFIELD CLIN FDN MED RES & EDUC,MARSHFIELD,WI 54449
来源：
HUMAN GENETICS | 1991年 / 87卷 / 04期
关键词：
D O I：
暂无
中图分类号：
Q3 [遗传学];
学科分类号：
071007 ; 090102 ;
摘要：
An (AC)n repeat within the anonymous DNA sequence D21S171 was shown to be highly polymorphic in members of the 40 Centre d'Etude du Polymorphisme Humain (CEPH) families. Ten different alleles at this marker locus were detected by electrophoresis on polyacrylamide gels of DNA amplified by the polymerase chain reaction (PCR) using primers flanking the (AC)n repeat. The observed heterozygosity was 66%. PCR amplification of DNA from somatic cell hybrids mapped D21S171 to human chromosome 21, and linkage analysis localized this marker close to the loci CD18, PFKL, D21S113 and D21S112 in chromosomal band 21q22.3. In CEPH family 12 a de novo allele has been observed in a maternally derived chromosome.
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页码：401 / 404
页数：4
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