AN INDUCIBLE EXPRESSION SYSTEM FOR THE PRODUCTION OF HUMAN LACTOFERRIN IN ASPERGILLUS-NIDULANS

被引：41
|
作者：
WARD, PP
MAY, GS
HEADON, DR
CONNEELY, OM
机构：
[1] BAYLOR COLL MED,DEPT CELL BIOL,1 BAYLOR PLAZA,HOUSTON,TX 77030
[2] NATL UNIV IRELAND UNIV COLL GALWAY,DEPT BIOCHEM,CELL & MOLEC BIOL GRP,GALWAY,IRELAND
来源：
GENE | 1992年 / 122卷 / 01期
关键词：
RECOMBINANT DNA; IRON-BINDING GLYCOPROTEIN; FILAMENTOUS FUNGI; ALCA PROMOTER; SECRETION; FUNCTIONAL ASSAY;
D O I：
10.1016/0378-1119(92)90054-S
中图分类号：
Q3 [遗传学];
学科分类号：
071007 ; 090102 ;
摘要：
The production and secretion of human lactoferrin (hLF) in Aspergillus nidulans is described. The hLF cDNA was expressed under the control of the strong ethanol-inducible alcohol dehydrogenase (alcA) promoter. Recombinant hLF (re-hLF) is produced at levels up to 5 mug/ml. Approximately 30% of the re-hLF produced in this system is secreted into the growth medium. The re-hLF is indistinguishable from native hLF with respect to size and immunoreactivity. Furthermore, re-hLF is functional by the criterion of iron-binding capacity. The A. nidulans expression system offers an inexpensive, convenient method for the controlled production of mg amounts of biologically active mammalian glycoproteins.
引用
收藏
页码：219 / 223
页数：5
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