BOVINE PARATHYROID CELLS - CULTURES MAINTAINED FOR MORE THAN 140 POPULATION DOUBLINGS

被引：63
作者：
BRANDI, ML
FITZPATRICK, LA
COON, HG
AURBACH, GD
机构：
[1] NIADDKD, METAB DIS BRANCH, ENDOCRINE REGULAT SECT, BETHESDA, MD 20205 USA
[2] NCI, DIV CANC BIOL & DIAG, GENET LAB, BETHESDA, MD 20205 USA
来源：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1986年 / 83卷 / 06期
关键词：
D O I：
10.1073/pnas.83.6.1709
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
Primary cultures of bovine parathyroid cells were developed using Coon''s modified Ham''s F-12 medium containing low (0.3 mM) concentrations of calcium and supplements of bovine hypothalamic extract, bovine pituitary extract, epidermal growth factor, insulin, transferrin, selenous acid, hydrocortisone, triiodothyronine, retinoic acid, and galactose. These cells were cultured serially on serum-coated dishes for 140 population doublings before signs of senescence were detected. The cells were epithelioid and maintained a high degree of differentiation as evidenced by calcium regulation of both growth and secretion and by prostaglandin E1 stimulation of cAMP formation and hormone release.
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页码：1709 / 1713
页数：5
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