ALTERATION OF HUMAN MYOGLOBIN PROXIMAL HISTIDINE TO CYSTEINE OR TYROSINE BY SITE-DIRECTED MUTAGENESIS - CHARACTERIZATION AND THEIR CATALYTIC ACTIVITIES

被引：75

作者：

ADACHI, S ^{[1
]}

NAGANO, S ^{[1
]}

WATANABE, Y ^{[1
]}

ISHIMORI, K ^{[1
]}

MORISHIMA, I ^{[1
]}

机构：

[1] KYOTO UNIV,GRAD SCH ENGN,DIV MOLEC ENGN,KYOTO 606,JAPAN

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1991年 / 180卷 / 01期

关键词：

D O I：

10.1016/S0006-291X(05)81266-5

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Two mutant proteins of human myoglobin (Mb) that exhibit altered axial ligations were prepared by site-directed mutagenesis of a cloned gene for human Mb. The normal axial ligand residue, histidine 93(F8), was replaced with cysteine or tyrosine, resulting in H93C or H93Y Mb, respectively. Cysteine or tyrosine coordination to the ferric heme iron is verified by electronic absorption, 1H-NMR, EPR spectra, and redox potentials of Fe2+/Fe3+ couple. Their mono-oxygenation activities of styrene are also discussed. © 1991 Academic Press, Inc.

引用

页码：138 / 144

页数：7

共 27 条

[21] STUDIES ON EPR AND LIGHT ABSORPTION SPECTRA OF HORSE ERYTHROCYTE CATALASE AND ITS DERIVATIVES [J].

TORII, K ;

OGURA, Y .

JOURNAL OF BIOCHEMISTRY, 1968, 64 (02) :171-&

[22] SPIN-STATE CHANGES IN CYTOCHROME-P-450CAM ON BINDING OF SPECIFIC SUBSTRATES [J].

TSAI, R ;

YU, CA ;

GUNSALUS, IC ;

PEISACH, J ;

BLUMBERG, W ;

ORMEJOHN.WH ;

BEINERT, H .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1970, 66 (04) :1157-&

[23] ELECTROSTATIC INTERACTIONS IN WILD-TYPE AND MUTANT RECOMBINANT HUMAN MYOGLOBINS [J].

VARADARAJAN, R ;

LAMBRIGHT, DG ;

BOXER, SG .

BIOCHEMISTRY, 1989, 28 (09) :3771-3781

[24] CLONING, EXPRESSION IN ESCHERICHIA-COLI, AND RECONSTITUTION OF HUMAN MYOGLOBIN [J].

VARADARAJAN, R ;

SZABO, A ;

BOXER, SG .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1985, 82 (17) :5681-5684

[25]

WILLIAMS RJP, 1974, BIOCHEM MEDICA, P183

[26] EFFECTS OF 2,4-SUBSTITUENTS OF DEUTEROHEME UPON REDOX POTENTIALS OF HORSERADISH PEROXIDASES [J].

YAMADA, H ;

MAKINO, R ;

YAMAZAKI, I .

ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 1975, 169 (01) :344-353

[27]

YU CA, 1974, J BIOL CHEM, V249, P94

← 1 2 3 →