DESORPTION OF PROTEINS FROM THE AIR WATER INTERFACE

被引:40
作者
MACRITCHIE, F
机构
[1] CSIRO, Wheat Research Unit, North, Ryde, Aust, CSIRO, Wheat Research Unit, North Ryde, Aust
关键词
DESORPTION;
D O I
10.1016/0021-9797(85)90353-4
中图分类号
O64 [物理化学(理论化学)、化学物理学];
学科分类号
070304 ; 081704 ;
摘要
Rates of desorption of proteins were measured by the decrease in area at a reference surface pressure of 5 mN m** minus **1 after maintaining monolayers at higher constant pressures for given times. Kinetics followed a t** one-half relation, consistent with a diffusion-controlled process from a subsurface layer of constant concentration. For two proteins studied, beta -lactoglobulin and insulin, initial rates of desorption from mixed monolayers with nondesorbable components were the same as for the pure monolayers. A comparison of five proteins showed that rates of desorption decreased markedly with increasing molecular weight at a given surface pressure. These results should assist in understanding transport processes across interfaces and membranes and competitive adsorption from protein mixtures in processes such as blood clotting.
引用
收藏
页码:119 / 123
页数:5
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