TRANSCRIPTION-DEPENDENT RECOMBINATION INDUCED BY TRIPLE-HELIX FORMATION

被引：63

作者：

KOHWI, Y

PANCHENKO, Y

机构：

[1] La Jolla Cancer Research Foundation, La Jolla

来源：

GENES & DEVELOPMENT | 1993年 / 7卷 / 09期

关键词：

RECOMBINATION; TRANSCRIPTION; NEGATIVE SUPERCOILING; DNA STRUCTURE; TRIPLE HELIX;

D O I：

10.1101/gad.7.9.1766

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

The homologous recombination between direct repeat sequences separated by either 200 or 1000 bp was induced by active transcription of the downstream gene when poly(dG)-poly(dC) sequences exist between the two direct repeats. This dG tract-mediated and transcription-induced recombination was RecA independent, and the frequency of recombination was dependent on both the length and the orientation of the poly(dG)-poly(dC) sequences relative to the gene. An intramolecular dG.dG.dC triplex formation was detected in Escherichia coli cells in a length-dependent manner when the transcription of the downstream gene was activated. We suggest that the negative superhelical strain generated by active transcription of the downstream gene induces poly(dG)-poly(dC) sequences to adopt a triple-helix structure in vivo and that this structure brings two remote sequences together to stimulate homologous recombination.

引用

页码：1766 / 1778

页数：13

共 46 条

[1] ON THE FORMATION OF SPONTANEOUS DELETIONS - THE IMPORTANCE OF SHORT SEQUENCE HOMOLOGIES IN THE GENERATION OF LARGE DELETIONS [J].