INHIBITION OF HERPES-SIMPLEX VIRUS-1 REPRODUCTION WITH HYDROPHOBIZED ANTISENSE OLIGONUCLEOTIDES

被引：7
作者：
VINOGRADOV, SV
SUZDALTSEVA, Y
ALAKHOV, VY
KABANOV, AV
机构：
[1] MOSCOW INST BIOTECHNOL INC,MOSCOW 119899,RUSSIA
[2] MOSCOW MV LOMONOSOV STATE UNIV,FAC CHEM,MOSCOW 119899,RUSSIA
来源：
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1994年 / 203卷 / 02期
关键词：
D O I：
10.1006/bbrc.1994.2275
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Antisense oligonucleotides were modified by their ends with hydrophobic substituents which permitted enhancing their activity. The effect of hydrophobized oligonucleotides on reproduction of Herpes Simplex Virus type 1 in Vero cells was studied. Two types of oligonucleotides were used: a 12-mer complementary to the splicing site 983-994 of early mRNA-5 of the virus and 19-mer complementary to the site of mRNA that encodes the virus DNA-polymerase. These oligonucleotides were modified by 5'-ends with n-undecyl or cholesteryl moieties and by 3'-ends with acridine or 1,7-heptanediol. In comparison with the unnmodified oligonucleotides the hydrophobized ones were significantly more active and inhibited HSV-1 infection at micromolar concentrations. (C) 1994 Academic Press, Inc.
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页码：959 / 966
页数：8
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