GENE-EXPRESSION OF PROTEIN PHOSPHATASES IN RAT ASCITES HEPATOMA-CELL LINES

被引：0

作者：

SAADAT, M ^{[1
]}

KITAMURA, K ^{[1
]}

MIZUNO, Y ^{[1
]}

SAEKI, H ^{[1
]}

KUDO, T ^{[1
]}

KIKUCHI, K ^{[1
]}

机构：

[1] HOKKAIDO UNIV,INST IMMUNOL SCI,BIOCHEM SECT,KITA 15,NISHI 7,KITA KU,SAPPORO,HOKKAIDO 060,JAPAN

来源：

CANCER DETECTION AND PREVENTION | 1994年 / 18卷 / 02期

关键词：

PROTEIN PHOSPHATASE; GENE EXPRESSION; ASCITES HEPATOMA; GROWTH RATE; HARVEST TIME;

D O I：

暂无

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

Gene expression of protein phosphatases 1alpha, 1gamma1, 2Aalpha, and 2Calpha in 14 rat ascites hepatoma cell lines was studied by Northern blot hybridization. The expression of PP1alpha and PP2Calpha was increased and decreased, respectively, in all of the ascites hepatoma (AH) cells compared to rat liver, whereas the expression of PP1gamma1 and PP2Aalpha was increased in about 50% of them. Relative gene expression was affected by several factors, such as harvest time, transplantation rate, percentage of free cells, and sex; the first factor was more important than the others. Relative gene expression of PP1alpha had a negative correlation with harvest time, whereas gene expression of PP1gamma1 and PP2Aalpha had a nonlinear (hyperbolic) correlation with harvest time. We suggest that there is a relationship between growth rate and expression of protein phosphatase genes. Our data also suggest that PP1gamma1 mRNA is positively controlled by PP2Aalpha mRNA.

引用

页码：115 / 122

页数：8