THE SEQUENCE AND GENOMIC ORGANIZATION OF THE HUMAN TYPE-2 ANGIOTENSIN-II RECEPTOR

被引：63
作者：
MARTIN, MM [1 ]
ELTON, TS [1 ]
机构：
[1] UNIV ALABAMA,VASC BIOL & HYPERTENS PROGRAM,BIRMINGHAM,AL 35294
来源：
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1995年 / 209卷 / 02期
关键词：
D O I：
10.1006/bbrc.1995.1537
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
A human genomic DNA library was screened utilizing a human angiotensin II type 2 receptor (hAT(2)R) cDNA as a probe. Several positive clones were isolated and characterized. A comparison of the hAT(2)R cDNA sequence with the hAT(2)R genomic clone sequence suggests that the hAT(2)R gene is composed of three exons and spans at least 5 kb. Exons 1 and 2 encode for 5' untranslated mRNA sequence and exon 3 harbors the entire uninterrupted open reading frame of the hAT(2)R. Sequence analysis of the 5'-flanking region of the hAT(2)R gene demonstrates that it contains the typical sequence motifs found in many eukaryotic promoters. Interestingly, however, this promoter region also includes an interferon consensus sequence binding protein site (ICSBP) and a putative embryonal, long terminal repeat binding protein (ELF) site. The presence of these novel putative transcription factor binding sites suggests that this gene may be regulated in a unique manner. (C) 1995 Academic Press. Inc.
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页码：554 / 562
页数：9
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