ROLE OF HUMAN T-CELL LEUKEMIA-VIRUS TYPE-1 X-REGION PROTEINS IN IMMORTALIZATION OF PRIMARY HUMAN-LYMPHOCYTES IN CULTURE

被引：278

作者：

GRASSMANN, R ^{[1
]}

BERCHTOLD, S ^{[1
]}

RADANT, I ^{[1
]}

ALT, M ^{[1
]}

FLECKENSTEIN, B ^{[1
]}

SODROSKI, JG ^{[1
]}

HASELTINE, WA ^{[1
]}

RAMSTEDT, U ^{[1
]}

机构：

[1] HARVARD UNIV,SCH MED,DANA FARBER CANC INST,DIV HUMAN RETROVIROL,BOSTON,MA 02115

来源：

JOURNAL OF VIROLOGY | 1992年 / 66卷 / 07期

关键词：

D O I：

10.1128/JVI.66.7.4570-4575.1992

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Human T-cell leukemia virus type 1 (HTLV-1) immortalizes human CD4+ T lymphocytes in culture. Previous studies show that in the context of a herpesvirus saimiri vector, the sequence of the X region at the 3' end of the HTLV-1 genome is also capable of immortalizing CD4+ lymphocytes in the absence of HTLV-1 structural proteins. The X region of HTLV-1 encodes two trans-acting viral proteins, the 42-kDa Tax protein and the 27-kDa Rex protein. Infection of human cord blood cells with herpesvirus saimiri recombinants which contain HTLV-1 X region sequences defective for expression of tax, rex, or both tax and rex demonstrates that tax function is necessary and sufficient for immortalization of primary human CD4+ cord blood lymphocytes in culture in the context of the herpesvirus saimiri vector.

引用

页码：4570 / 4575

页数：6

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