A fiber-optic system for dual-modality photoacoustic microscopy and confocal fluorescence microscopy using miniature components

被引:40
作者
Chen, Sung-Liang [1 ]
Xie, Zhixing [1 ]
Guo, L. Jay [2 ]
Wang, Xueding [1 ]
机构
[1] Univ Michigan, Dept Radiol, Ann Arbor, MI 48109 USA
[2] Univ Michigan, Dept Elect Engn & Comp Sci, Ann Arbor, MI 48109 USA
基金
美国国家卫生研究院; 美国国家科学基金会;
关键词
Photoacoustic microscopy; Confocal fluorescence microscopy; Microring resonator; Microelectromechanical systems; Endoscopy; Bladder cancer;
D O I
10.1016/j.pacs.2013.07.001
中图分类号
O42 [声学];
学科分类号
070206 ; 082403 ;
摘要
Imaging of the cells and microvasculature simultaneously is beneficial to the study of tumor angiogenesis and microenvironments. We designed and built a fiber-optic based photoacoustic microscopy (PAM) and confocal fluorescence microscopy (CFM) dual-modality imaging system. To explore the feasibility of this all-optical device for future endoscopic applications, a microelectromechanical systems (MEMS) scanner, a miniature objective lens, and a small size optical microring resonator as an acoustic detector were employed trying to meet the requirements of miniaturization. Both the lateral resolutions of PAM and CFM were quantified to be 8.8 mu m. Axial resolutions of PAM and CFM were experimentally measured to be 19 mu m and 53 mu m, respectively. The experiments on ex vivo animal bladder tissues demonstrate the good performance of this system in imaging not only microvasculature but also cellular structure, suggesting that this novel imaging technique holds potential for improved diagnosis and guided treatment of bladder cancer. (C) 2013 The Authors. Published by Elsevier GmbH. Open access under CC BY license.
引用
收藏
页码:30 / 35
页数:6
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