STRUCTURAL CHARACTERIZATION OF BACITRACIN COMPONENTS BY FRIT-FAST ATOM BOMBARDMENT (FAB) LIQUID-CHROMATOGRAPHY MASS-SPECTROMETRY (LC/MS)

被引：22

作者：

IKAI, Y ^{[1
]}

OKA, H ^{[1
]}

HAYAKAWA, J ^{[1
]}

HARADA, K ^{[1
]}

SUZUKI, M ^{[1
]}

机构：

[1] MEIJO UNIV,FAC PHARM,TEMPAKU KU,NAGOYA,AICHI 468,JAPAN

来源：

JOURNAL OF ANTIBIOTICS | 1992年 / 45卷 / 08期

关键词：

D O I：

10.7164/antibiotics.45.1325

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The structural characterization of minor components of bacitracin (BC) complex was carried out using a technique of liquid chromatography/mass spectrometry (LC/MS). Satisfactory total ion current chromatogram of BC complex and excellent mass spectra of many components were given by Frit-fast atom bombardment (FAB) LC/MS analytical system, and the structures of 13 minor components could be proposed. The 13 minor components were classified into two groups, bacitracin A (BC-A) related components and bacitracin F (BC-F) related components depending on their common N-terminal moieties. The structures of BC-A related components and BC-F related components were the same as those of BC-A and BC-F, respectively, except that one to three of isoleucine and leucine residues are replaced by valines. The BC-F related components were degradation products of BC-A related components through the same degradation process as that of BC-A.

引用

页码：1325 / 1334

页数：10

共 26 条

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