IMMUNOELECTRON MICROSCOPIC LOCALIZATION OF ONE OF THE SPORE GERMINATION PROTEINS, GERAB, IN BACILLUS-SUBTILIS SPORES

被引：20
|
作者：
SAKAE, Y [1 ]
YASUDA, Y [1 ]
TOCHIKUBO, K [1 ]
机构：
[1] NAGOYA CITY UNIV,SCH MED,DEPT MICROBIOL,MIZUHO KU,NAGOYA,AICHI 467,JAPAN
来源：
JOURNAL OF BACTERIOLOGY | 1995年 / 177卷 / 21期
关键词：
D O I：
10.1128/jb.177.21.6294-6296.1995
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
Ultrastructural localization of GerAB, one of the proteins of Bacillus subtilis spores related to L-alanine-initiated germination, was investigated by immunoelectron microscopy with antipeptide (residues 61 to 80 of GerAB) antiserum and a colloidal gold-immnnoglobulin G complex. Immunogold particles were visualized in the boundary region between the cortex and coat of dormant spores, and they were broadly dispersed into the cortex region after germination.
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收藏
页码：6294 / 6296
页数：3
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