IMMUNOCHEMICAL DETECTION OF THE MINERALOCORTICOID RECEPTOR IN RAT-BRAIN

被引：38

作者：

AGARWAL, MK ^{[1
]}

MIRSHAHI, F ^{[1
]}

MIRSHAHI, M ^{[1
]}

ROSTENE, W ^{[1
]}

机构：

[1] UNIV PARIS 07,CTR UNIV CORDELIERS,F-75221 PARIS 05,FRANCE

来源：

NEUROENDOCRINOLOGY | 1993年 / 58卷 / 05期

关键词：

MINERALOCORTICOIDS; RECEPTORS; SPIROLACTONES; BRAIN; IMMUNOHISTOCHEMISTRY;

D O I：

10.1159/000126593

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

The mineralocorticoid receptor (MCR) in the brain of adult male rats was analyzed with the aid of an antiserum generated by immunizing rabbits with this protein purified biochemically from rat kidney. In Western blots, the antibody recognized a single band of protein of about 98 kD from all target tissues studied to date. The granular cells in the cerebellum appeared to be the richest region of the central nervous system in the MCR analyzed by dot blots and by immunoperoxidase staining. High immunoreactivity was also observed in the dorsal and ventral hippocampus, as well as the anterior, mediobasal and posterior hypothalamus. In contrast, midbrain, septum and striatum were MCR-negative. The antibody macroaggregated and precipitated brain MCR labelled with H-3-aldosterone but did not recognize H-3-RU 26752 bound nonspecifically to non-MCR proteins in whole brain cytosol. Thus, MCR function and expression in the adult rat brain need to be reassessed.

引用

页码：575 / 580

页数：6

共 24 条

[1] PARADOXICAL DIFFERENCES IN THE RECEPTOR-BINDING OF 2 NEW ANTIMINERALOCORTICOIDS [J].

AGARWAL, MK ;

KALIMI, M .

BIOCHIMICA ET BIOPHYSICA ACTA, 1988, 964 (01) :105-112

[2]

AGARWAL MK, 1991, RENAL PHYSIOL BIOCH, V14, P217

[3] RAT LUNG POSSESSES THE MINERALOCORTICOID RECEPTOR [J].

AGARWAL, MK ;

MIRSHAHI, M .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1992, 183 (02) :405-410

[4] TYPE-I CORTICOSTEROID RECEPTOR-LIKE IMMUNOREACTIVITY IN THE RAT CNS - DISTRIBUTION AND REGULATION BY CORTICOSTEROIDS [J].

AHIMA, R ;

KROZOWSKI, Z ;

HARLAN, R .

JOURNAL OF COMPARATIVE NEUROLOGY, 1991, 313 (03) :522-538

[5] CLONING OF HUMAN MINERALOCORTICOID RECEPTOR COMPLEMENTARY-DNA - STRUCTURAL AND FUNCTIONAL KINSHIP WITH THE GLUCOCORTICOID RECEPTOR [J].

ARRIZA, JL ;

WEINBERGER, C ;

CERELLI, G ;

GLASER, TM ;

HANDELIN, BL ;

HOUSMAN, DE ;

EVANS, RM .

SCIENCE, 1987, 237 (4812) :268-275

[6] THE NEURONAL MINERALOCORTICOID RECEPTOR AS A MEDIATOR OF GLUCOCORTICOID RESPONSE [J].

ARRIZA, JL ;

SIMERLY, RB ;

SWANSON, LW ;

EVANS, RM .

NEURON, 1988, 1 (09) :887-900

[7] CHARACTERIZATION OF RAT-BRAIN ALDOSTERONE RECEPTORS REVEALS HIGH-AFFINITY FOR CORTICOSTERONE [J].

BEAUMONT, K ;

FANESTIL, DD .

ENDOCRINOLOGY, 1983, 113 (06) :2043-2051

[8] GRANULE CELL GENESIS IN THE HIPPOCAMPUS OF RATS TREATED NEONATALLY WITH HYDROCORTISONE [J].

BOHN, MC .

NEUROSCIENCE, 1980, 5 (11) :2003-+

[9]

BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3

[10] FEEDBACK ACTION AND TONIC INFLUENCE OF CORTICOSTEROIDS ON BRAIN-FUNCTION - A CONCEPT ARISING FROM THE HETEROGENEITY OF BRAIN RECEPTOR SYSTEMS [J].

DEKLOET, ER ;

REUL, JMHM .

PSYCHONEUROENDOCRINOLOGY, 1987, 12 (02) :83-105

← 1 2 3 →