A SIMPLE AND SENSITIVE ENZYME-MEDIATED ASSAY OF BIOTIN

被引：0
作者：
HUANG, ZJ
HAUGLAND, RP
SZALECKA, D
HAUGLAND, RP
机构：
来源：
BIOTECHNIQUES | 1992年 / 13卷 / 04期
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中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Free biotin was quantitated by a competition by coating biotin-bovine serum albumin conjugate on a polystyrene microplate for binding to avidin-beta-galactosidase conjugate. The enzyme conjugate remaining on the plate surface as a result of the competition was detected by reaction with one of the following fluorogenic substrates, resorufin beta-D-galactoside and fluorescein di-beta-D-galactoside, in a fluorescence plate reader. Free biotin as little as 0.1 nmol can be routinely detected.
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页码：543 / 546
页数：4
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