CONSTRUCTION OF BRANCHED DNA (BDNA) MOLECULES BY CHEMICAL LIGATION

被引：7
|
作者：
DOLINNAYA, N
GRYAZNOV, S
AHLE, D
CHANG, CA
SHABAROVA, ZA
URDEA, MS
HORN, T
机构：
[1] NORTHWESTERN UNIV, DEPT CHEM, EVANSTON, IL 60208 USA
[2] MOSCOW MV LOMONOSOV STATE UNIV, DEPT CHEM, MOSCOW 119899, RUSSIA
来源：
BIOORGANIC & MEDICINAL CHEMISTRY LETTERS | 1994年 / 4卷 / 08期
关键词：
D O I：
10.1016/S0960-894X(01)80672-7
中图分类号：
R914 [药物化学];
学科分类号：
100701 ;
摘要：
Chemical ligation has been investigated in the assembly of branched DNA (bDNA) molecules. Water soluble coupling reagents were used to ligate single-stranded linear oligomers to bDNA molecules containing 5 and 15 branches. The ligations were particularly efficient when one of the oligomers was partially double-stranded constructed by branching to create a terminal ligation linker (AutoLink). Branched DNA molecules containing more than 1000 nucleotides were assembled.
引用
收藏
页码：1011 / 1018
页数：8
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