RAPID SCREENING FOR CELL-SURFACE BINDING MONOCLONAL-ANTIBODIES

被引：0
|
作者：
WHEELOCK, MJ
机构：
[1] University of Toledo, Toledo, OH
来源：
BIOTECHNIC & HISTOCHEMISTRY | 1991年 / 66卷 / 02期
关键词：
ANTIBODY; MONOCLONAL; SURFACE PROTEINS; IMMUNOFLUORESCENCE; WESTERN BLOT;
D O I：
10.3109/10520299109110560
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
A procedure is described which allows for rapid detection of cell surface binding or cytoskeleton binding monoclonal antibodies. At the same time this procedure ensures that selected antibodies will be useful in Western blot analysis. This procedure including the cytochemistry and Western blot analysis requires only 100-mu-l of supernatant and can be done directly from the original 96 well plates into which the fusion was plated. One person can easily assay several hundred supernatants in one day for the ability to stain both cells and selected proteins in Western blot analysis.
引用
收藏
页码：102 / 108
页数：7
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