RAS ANTAGONIZES CAMP-STIMULATED GLUCAGON GENE-TRANSCRIPTION IN PANCREATIC-ISLET CELL-LINES

被引：7

作者：

GHERZI, R

BRIATA, P

FEHMANN, HC

GOKE, B

机构：

[1] IST NAZL RIC CANC, CELL BIOL LAB, I-16132 GENOA, ITALY

[2] INST MOLEK BIOL & TUMORFORSCH, D-35033 MARBURG, GERMANY

来源：

FEBS LETTERS | 1994年 / 353卷 / 03期

关键词：

RAS; GLUCAGON GENE; GENE TRANSCRIPTION; INSULINOMA; GLUCAGONOMA; PROTEIN KINASE A;

D O I：

10.1016/0014-5793(94)01050-1

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Ras, a GTP-binding protein, converts membrane tyrosine kinase signalling to changes in gene expression patterns. Utilising a rat glucagon promoter-CAT construct (p[-1.1]GLU-CAT) we demonstrate in transient transfection experiments that the oncogenic Ras inhibits cAMP-dependent activation of p[-1.1]GLU-CAT in both glucagonoma InR 1-G9 and insulinoma beta-TCl cells. Conversely, the expression of a dominant negative mutant of Ras enhances the cAMP-induced activation of p[-1.1]GLU-CAT transcription in these cells. Our data suggests a functional interference of Ras with the cAMP-dependent transcription of the glucagon gene.

引用

页码：277 / 280

页数：4