MOLECULAR-GENETIC PROPERTIES OF THE YEAST TORULASPORA PRETORIENSIS - CHARACTERIZATION OF CHROMOSOMAL DNA AND GENETIC-TRANSFORMATION BY SACCHAROMYCES-CEREVISIAE-BASED PLASMIDS

被引：10

作者：

ODA, Y

TONOMURA, K

机构：

[1] Department of Food Science and Technology, Fukuyama University, Fukuyama, Hiroshima

来源：

CURRENT GENETICS | 1995年 / 27卷 / 02期

关键词：

CHROMOSOMAL DNA; TORULASPORA PRETORIENSIS; TRANSFORMATION; HYBRIDIZATION;

D O I：

10.1007/BF00313426

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

Chromosomal DNA banding patterns were obtained for three strains of Torulaspora pretoriensis by contour-clamped homogenous-electric-field gel electrophoresis. Chromosomes were resolved into six or seven bands in the range of 800 to 2000 kb, and a polymorphism of these lengths was observed. By Southern-blot analysis, the three strains were shown to lack the DNA sequences homologous to the URA3, LEU2, TRP1, and HO genes of Saccharomyces cerevisiae. A uracil auxotrophic mutant derived from T. pretoriensis was transformed with three plasmids (YEp24, YRpHI, and YCp50) carrying the URA3 gene of S. cerevisiae by the lithium acetate method.

引用

页码：131 / 134

页数：4