REDUCTION OF GLUCOSE INTERFERENCE IN BIOCHEMICAL ANALYSES BASED ON INFRARED-SPECTROSCOPY

Simultaneous analyses of biochemical components in human serum were investigated using attenuated total reflection (ATR) and FT-IR in combination with the partial least squares (PLS) calibration method. When the glucose concentration in a sample was out of range of that in the standard solutions for calibration, measurements of biochemical components were found to be affected by the glucose concentration. Glucose interference could be reduced by optimizing the number of standard solutions and the range of glucose concentrations in the standard solutions. When the optimized standard solutions were used for calibration, good correlations between the ATR/FT-IR method and the automatic clinical analyzer could be obtained for measurements of triglycerides, urea, cholesterol, total protein and glucose, while correlation coefficients for uric acid and creatinine were 0.77 and 0.85 respectively because of insufficient sensitivity of the detector.