THE EFFECT OF ACARBOSE ON THE INTESTINAL METABOLISM OF GLUCOSE IN-VITRO

The effect of acarbose on the intestinal metabolism of glucose was investigated using an in vitro perfused preparation of the isolated rat small intestine-pancreas. In preparations perfused without intraluminal sucrose administration, the total glucose recovered in the portal effluent and the portal values of lactate, pyruvate and alanine did not depend on whether or not acarbose [1.5 mg/kg body weight (b.w.)] was present in the intestinal lumen. The intestinal glucose and lactate contents were very low at the end of the experiment, and identical with or without acarbose. Insulin and glucagon concentrations remained constant during the whole perfusion period. After intraluminal administration of sucrose a clear increase in portal glucose concentration was observed, which was severely reduced by acarbose administration; no changes in portal levels of lactate, pyruvate, alanine, insulin and glucagon were observed. The intestinal content of sucrose at the end of the study was significantly higher in the presence of acarbose (1.5 mg/kg b.w.), while the glucose concentration was low both with and without acarbose (0.20 +/- 0.08 vs 0.29 +/- 0.09 mmol/l respectively). These results suggest that acarbose does not influence the metabolic utilization of the glucose being translocated from the lumen.