LIPASE REACTION IN AOT-ISOOCTANE REVERSED MICELLES - EFFECT OF WATER ON EQUILIBRIA

被引：50

作者：

HAN, D

RHEE, JS

LEE, SB

机构：

[1] KOREA ADV INST SCI & TECHNOL,DEPT BIOL SCI & ENGN,POB 150,SEOUL 131,SOUTH KOREA

[2] KOREA ADV INST SCI & TECHNOL,CTR GENET ENGN,SEOUL 131,SOUTH KOREA

来源：

BIOTECHNOLOGY AND BIOENGINEERING | 1987年 / 30卷 / 03期

关键词：

BIOCHEMICAL ENGINEERING - CHEMICAL REACTIONS - Hydrolysis - FATTY ACIDS - Biosynthesis - GLYCEROL - Biosynthesis - VEGETABLE OILS - Hydrolysis;

D O I：

10.1002/bit.260300309

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Equations corresponding to a two-substrate, second-order reversible model are derived and used for further analysis. The progress curves predicted from the rate equations agree very well with the experimental results under various reaction conditions. The values of the molar ratio of water to surfactant (R) which maximize the initial reaction rate and maximum fractional conversion is predictable from the derived rate equations and the resulting relationship between R and the kinetic constants. The effect of water on equilibria for hydrolytic reaction in reversed micelles has been investigated using lipase as a model enzyme.

引用

页码：381 / 388

页数：8

共 18 条

[1] MICELLAR SOLUBILIZATION OF BIO-POLYMERS IN ORGANIC-SOLVENTS .5. ACTIVITY AND CONFORMATION OF ALPHA-CHYMOTRYPSIN IN ISOOCTANE-AOT REVERSE MICELLES [J].