MYCOBACTERIUM-PARATUBERCULOSIS ANTIGEN-D - CHARACTERIZATION AND EVIDENCE THAT IT IS A BACTERIOFERRITIN

被引：34

作者：

BROOKS, BW ^{[1
]}

YOUNG, NM ^{[1
]}

WATSON, DC ^{[1
]}

ROBERTSON, RH ^{[1
]}

SUGDEN, EA ^{[1
]}

NIELSEN, KH ^{[1
]}

BECKER, SAWE ^{[1
]}

机构：

[1] NATL RES COUNCIL CANADA,DIV BIOL SCI,OTTAWA K1A 0R6,ONTARIO,CANADA

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 1991年 / 29卷 / 08期

关键词：

D O I：

10.1128/JCM.29.8.1652-1658.1991

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

By using a combination of agarose and polyacrylamide gel electrophoresis, Mycobacterium paratuberculosis antigen D was resolved from a crude sonicated preparation of the organism and characterized as a component with a molecular mass of approximately 400,000 Da. While this component was composed mainly of protein, with unusually high proportions of glutamic acid and leucine, it was resistant to digestion with a number of proteolytic enzymes. Structural detail revealed by electron microscopy, amino acid sequence data, and the demonstration of a Soret band in its absorption spectrum indicated that antigen D was similar to an Escherichia coli bacterioferritin.

引用

页码：1652 / 1658

页数：7

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