Whole genome sequence of the emerging oomycete pathogen Pythium insidiosum strain CDC-B5653 isolated from an infected human in the USA

被引:16
作者
Ascunce, Marina S. [1 ,2 ]
Huguet-Tapia, Jose C. [1 ]
Braun, Edward L. [3 ,4 ]
Ortiz-Urquiza, Almudena [5 ]
Keyhani, Nemat O. [5 ]
Goss, Erica M. [1 ,2 ]
机构
[1] Univ Florida, Emerging Pathogens Inst, Gainesville, FL USA
[2] Univ Florida, Dept Plant Pathol, Gainesville, FL 32611 USA
[3] Univ Florida, Dept Biol, Gainesville, FL USA
[4] Univ Florida, Genet Inst, Gainesville, FL USA
[5] Univ Florida, Dept Microbiol & Cell Sci, Gainesville, FL 32611 USA
基金
美国国家科学基金会;
关键词
Oomycete; Pythium insidiosum; Pythiosis; Human emerging pathogen; Genome sequencing;
D O I
10.1016/j.gdata.2015.11.019
中图分类号
Q3 [遗传学];
学科分类号
071007 ; 090102 ;
摘要
Pythium insidiosum ATCC 200269 strain CDC-B5653, an isolate from necrotizing lesions on the mouth and eye of a 2-year-old boy in Memphis, Tennessee, USA, was sequenced using a combination of Illumina MiSeq (300 bp paired-end, 14 millions reads) and PacBio (10 Kb fragment library, 356,001 reads). The sequencing data were assembled using SPAdes version 3.1.0, yielding a total genome size of 45.6 Mb contained in 8992 contigs, N-50 of 13 Kb, 57% G + C content, and 17,867 putative protein-coding genes. This Whole Genome Shotgun project has been deposited at DDBJ/EMBL/GenBank under the accession JRHR00000000. (c) 2015 The Authors. Published by Elsevier Inc.
引用
收藏
页码:60 / 61
页数:2
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