CHIRAL SEPARATION OF LEUCOVORIN WITH BOVINE SERUM-ALBUMIN USING AFFINITY CAPILLARY ELECTROPHORESIS

被引：140

作者：

BARKER, GE ^{[1
]}

RUSSO, P ^{[1
]}

HARTWICK, RA ^{[1
]}

机构：

[1] SUNY BINGHAMTON,DEPT CHEM,BINGHAMTON,NY 13902

来源：

ANALYTICAL CHEMISTRY | 1992年 / 64卷 / 23期

关键词：

D O I：

10.1021/ac00047a026

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A method for the determination of the (6R)- and (6S)-stereoisomers of leucovorin using electrokinetic chromatography (EKC) in the affinity mods has been developed. Bovine serum albumin (BSA) is used as a run buffer additive to incorporate enantiomeric selectivity into the system. Protein-wall interactions are minimized by using a poly(ethylene glycol) (PEG) coated capillary. Chiral resolution is obtained in 12.5 min with efficiencies greater than 200 000 theoretical plates using BSA as an additive, while no resolution is obtained in the absence of BSA. a general equation is derived to calculate the free energy of interaction between the leucovorin isomers and the BSA molecule. This method represents a new means of obtaining thermodynamic data for substrate binding interactions and for the general study of drug cross-reactions and interactions of drugs with serum and other proteins.

引用

页码：3024 / 3028

页数：5

共 33 条

[1] DIRECT LIQUID-CHROMATOGRAPHIC SEPARATION OF ENANTIOMERS ON IMMOBILIZED PROTEIN STATIONARY PHASES .3. OPTICAL RESOLUTION OF A SERIES OF N-AROYL D,L-AMINO ACIDS BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY ON BOVINE SERUM-ALBUMIN COVALENTLY BOUND TO SILICA [J].