METALLOANTIBODIES

被引：112

作者：

IVERSON, BL

IVERSON, SA

ROBERTS, VA

GETZOFF, ED

TAINER, JA

BENKOVIC, SJ

LERNER, RA

机构：

[1] SCRIPPS CLIN & RES FDN, DEPT CHEM, LA JOLLA, CA 92037 USA

[2] SCRIPPS CLIN & RES FDN, RES INST, LA JOLLA, CA 92037 USA

[3] PENN STATE UNIV, DEPT CHEM, UNIVERSITY PK, PA 16802 USA

来源：

SCIENCE | 1990年 / 249卷 / 4969期

关键词：

D O I：

10.1126/science.2116666

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

A metalloantibody has been constructed with a coordination site for metals in the antigen binding pocket. The Zn(II) binding site from carbonic anhydrase B was used as a model. Three histidine residues have been placed in the light chain complementarity determining regions of a single chain antibody molecule. In contrast to the native protein, the mutant displayed metal-dependent fluorescence-quenching behavior. This response was interpreted as evidence for metal binding in the three-histidine site with relative affinities in the order Cu(II) > Zn(II) > Cd(II). The presence of metal cofactors in immunoglobulins should facilitate antibody catalysis of redox and hydrolytic reactions.

引用

页码：659 / 662

页数：4

共 23 条

[1]

Benkovic SJ., 1990, CHEMTRACTS ORG CHEM, V3, P1

[2] SINGLE-CHAIN ANTIGEN-BINDING PROTEINS [J].