RATIONAL DESIGN OF AROMATIC POLYKETIDE NATURAL-PRODUCTS BY RECOMBINANT ASSEMBLY OF ENZYMATIC SUBUNITS

被引：230
作者：
MCDANIEL, R
EBERTKHOSLA, S
HOPWOOD, DA
KHOSLA, C
机构：
[1] STANFORD UNIV,DEPT CHEM ENGN,STANFORD,CA 94305
[2] JOHN INNES CTR,DEPT GENET,NORWICH NR4 7UH,NORFOLK,ENGLAND
来源：
NATURE | 1995年 / 375卷 / 6532期
关键词：
D O I：
10.1038/375549a0
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
Recent advances in understanding of bacterial aromatic polyketide biosynthesis allow the development of a set of design rules for the rational manipulation of chain synthesis, reduction of keto groups and early cyclization steps by genetic engineering. The concept of rational design is illustrated by the preparation of Streptomyces strains that produce two new polyketides by expression of combinations of appropriate enzymatic subunits from naturally occurring polyketide synthases. The potential for generating molecular diversity within this class of molecules by genetic engineering is enormous.
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页码：549 / 554
页数：6
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