CONSTRUCTION OF TRANSGENIC POTATO PLANTS RESISTANT TO POTATO-VIRUS-Y

被引：0
作者：
SOKOLOVA, MA
PUGIN, MM
SHULGA, OA
SKRYABIN, KG
机构：
来源：
MOLECULAR BIOLOGY | 1994年 / 28卷 / 05期
关键词：
POTATO VIRUS Y; TRANSGENIC PLANTS; VIRAL COAT PROTEIN; ANTISENSE RNA; PROTECTION BY GENE ENGINEERING;
D O I：
暂无
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Transgenic Belorusskii-3 potato plants were constructed expressing either potato virus Y (PVY) coat protein (CP) gene or its antisense mRNA. In some plants heterologous gene expression was enhanced by the 5'-untranslated leader of potato virus X inserted upstream of the PVY CP coding region. Plant resistance to PVY was examined by ELISA. Resistance to the PVY infection was lower in transgenic plants with weak expression of PVY CP mRNA and no detectable accumulation of PVY CP than in those with high expression of the PW CP mRNA and detectable PVY CP accumulation, or in those expressing the PVY CP antisense mRNA. We suppose that the resistance to PVY in our transgenic plant-virus system is mainly controlled by PVY CP mRNA
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页码：646 / 649
页数：4
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