PILI OF VIBRIO-CHOLERAE WIDELY DISTRIBUTED IN SEROGROUP-O1 STRAINS

被引：12

作者：

IWANAGA, M ^{[1
]}

NAKASONE, N ^{[1
]}

YAMASHIRO, T ^{[1
]}

HIGA, N ^{[1
]}

机构：

[1] UNIV RYUKYUS,SCH MED,COMPREHENS MED,NISHIHARA,OKINAWA 90301,JAPAN

来源：

MICROBIOLOGY AND IMMUNOLOGY | 1993年 / 37卷 / 01期

关键词：

VIBRIO-CHOLERAE O1; PILI; HEMAGGLUTINATION;

D O I：

10.1111/j.1348-0421.1993.tb03174.x

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

The distribution of Vibrio cholerae O1 pili consisting of 16 kDa subunit protein (16K-pili) was examined by Western blotting, using 211 strains from various origins and specific anti-16K-pili sera. The 16 kDa protein was detected in all 211 strains. The pili were purified from 3 El Tor and 3 classical strains, and characterized by hemagglutination and inhibition tests. All purified pili were hemagglutinative. However, the hemagglutinating activity of classical pili disappeared after exposure to 5 M urea and the agglutination induced by the classical pili was inhibited by D-mannose, alpha-methylmannoside, D-glucose and N-acetylglucosamine. On the contrary, El Tor pili were resistant to these sugars and urea.

引用

页码：23 / 28

页数：6

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