THE DISTRIBUTION OF GLUTAMATE RECEPTORS IN CULTURED RAT HIPPOCAMPAL-NEURONS - POSTSYNAPTIC CLUSTERING OF AMPA-SELECTIVE SUBUNITS

被引:316
作者
CRAIG, AM
BLACKSTONE, CD
HUGANIR, RL
BANKER, G
机构
[1] JOHNS HOPKINS UNIV, SCH MED, HOWARD HUGHES MED INST, BALTIMORE, MD 21205 USA
[2] JOHNS HOPKINS UNIV, SCH MED, DEPT NEUROSCI, BALTIMORE, MD 21205 USA
关键词
D O I
10.1016/0896-6273(93)90054-U
中图分类号
Q189 [神经科学];
学科分类号
071006 ;
摘要
The distribution of several glutamate receptor subunits was investigated in cultured rat hippocampal neurons by in situ hybridization and immunocytochemistry. The AMPA/kainate-selective receptors GluR1-6 exhibited two patterns of mRNA expression: most neurons expressed GluR1, R2, and R6, whereas only about 20% expressed significant levels of GluR3, R4, and R5. By immunocytochemistry, the metabotropic glutamate receptor mGluR1alpha was detectable only in a subpopulation of GABAergic interneurons. GluR1 and GluR2/3 segregated to the somatodendritic domain within the first week in culture, even in the absence of synaptogenesis. Glutamate receptor-enriched spines developed later and were present only on presumptive pyramidal cells, not on GABAergic interneurons. Clusters of GluR1 and GluR2/3 completely colocalized and were restricted to a subset of postsynaptic sites. Thus, glutamate receptor subunits exhibit both a cell type-specific expression and a selective subcellular localization.
引用
收藏
页码:1055 / 1068
页数:14
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