A MASS-SPECTROMETRIC SOLUTION TO THE ADDRESS PROBLEM OF COMBINATORIAL LIBRARIES

被引：133

作者：

BRUMMEL, CL ^{[1
]}

LEE, INW ^{[1
]}

ZHOU, Y ^{[1
]}

BENKOVIC, SJ ^{[1
]}

WINOGRAD, N ^{[1
]}

机构：

[1] PENN STATE UNIV,DEPT CHEM,152 DAVEY LAB,UNIV PK,PA 16802

来源：

SCIENCE | 1994年 / 264卷 / 5157期

关键词：

D O I：

10.1126/science.8153627

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The molecular weights of femtomole quantities of small peptides attached to polystyrene beads have been determined with imaging time-of-flight secondary ion mass spectrometry. The analysis is made possible by the selective clipping of the bond linking the peptide to a bead with trifluoroacetic acid vapor before the secondary ion mass spectrometry assay. The approach can be applied to large numbers of 30- to 60-micrometer polystyrene beads for the direct characterization of massive combinatorial libraries.

引用

页码：399 / 402

页数：4

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