SUSPENSION-CULTURE OF NICOTIANA-TABACUM CELLS IN A ROTARY-DRUM BIOREACTOR

被引：0

作者：

SHIBASAKI, N ^{[1
]}

HIROSE, K ^{[1
]}

YONEMOTO, T ^{[1
]}

TADAKI, T ^{[1
]}

机构：

[1] TOHOKU UNIV,DEPT BIOCHEM & ENGN,AOBA KU,SENDAI,MIYAGI 980,JAPAN

来源：

JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY | 1992年 / 53卷 / 04期

关键词：

NICOTIANA-TABACUM; SUSPENSION CULTURE; ROTARY-DRUM BIOREACTOR; AIRLIFT BIOREACTOR; ROLLER BOTTLE;

D O I：

暂无

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Suspension cultures of Nicotiana tabacum (tobacco) were studied using a rotary-drum bioreactor and the effects of various operating factors on culture growth were investigated. Conditions of oxygen concentration, aeration and drum rotation rates required for optimum culture growth rate were determined. The results were compared with those obtained in an air-lift bioreactor with a draft tube. The culture growth rates were higher in the rotary-drum bioreactor than in the air-lift bioreactor under the same conditions of aeration. The rotary-drum bioreactor was superior to the air-lift bioreactor in respect of oxygen mass transfer and the level of shear damage to N. tabacum cells.

引用

页码：359 / 363

页数：5

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