EXPRESSION OF A DNA METHYLATION (DAM) GENE IN ESCHERICHIA-COLI K-12

被引:10
作者
ARRAJ, JA [1 ]
WU, TH [1 ]
MARINUS, MG [1 ]
机构
[1] UNIV MASSACHUSETTS,SCH MED,DEPT PHARMACOL,55 LAKE AVE N,WORCESTER,MA 01655
关键词
D O I
10.1007/BF02092886
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Plasmid pMQ3, carrying the dam gene of Escherichia coli on a 6.1 Kb fragment, shows a tenfold increase in relative DNA adenine methylase activity, while plasmid p dam118, with a 1.14 Kb dam insert, shows only a twofold increase, although both plasmids were derived from plasmid pLC13-42. Since a copy number effect did not seem to be the cause of this difference, we have subcloned pMQ3 in order to determine whether the additional chromosomal DNA present in this plasmid is responsible for the enhancement of methylase activity. We show that the 346 base pairs upstream of dam contain sequences necessary for expression. DNA sequence analysis has revealed that in p dam118 only the 118 bases 5-prime to the dam gene are present in other constructs and that the additional upstream material is pBR322 DNA. This shows that p dam118 carries a DNA duplication. © 1990 Springer-Verlag New York Inc.
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页码:133 / 136
页数:4
相关论文
共 23 条
[1]   NUCLEOTIDE-SEQUENCE ANALYSIS OF THE CHLORAMPHENICOL RESISTANCE TRANSPOSON TN9 [J].
ALTON, NK ;
VAPNEK, D .
NATURE, 1979, 282 (5741) :864-869
[2]  
ARRAJ JA, 1983, J BACTERIOL, V152, P562
[3]   CHARACTERIZATION OF DNA ADENINE METHYLATION MUTANTS OF ESCHERICHIA-COLI-K12 [J].
BALE, A ;
DALARCAO, M ;
MARINUS, MG .
MUTATION RESEARCH, 1979, 59 (02) :157-165
[4]   THE ISOLATION AND CHARACTERIZATION OF THE ESCHERICHIA-COLI DNA ADENINE METHYLASE (DAM) GENE [J].
BROOKS, JE ;
BLUMENTHAL, RM ;
GINGERAS, TR .
NUCLEIC ACIDS RESEARCH, 1983, 11 (03) :837-851
[5]  
CARRAWAY M, 1987, GENETICS, V116, P343
[6]   INVITRO GENE FUSIONS THAT JOIN AN ENZYMATICALLY ACTIVE BETA-GALACTOSIDASE SEGMENT TO AMINO-TERMINAL FRAGMENTS OF EXOGENOUS PROTEINS - ESCHERICHIA-COLI PLASMID VECTORS FOR THE DETECTION AND CLONING OF TRANSLATIONAL INITIATION SIGNALS [J].
CASADABAN, MJ ;
CHOU, J ;
COHEN, SN .
JOURNAL OF BACTERIOLOGY, 1980, 143 (02) :971-980
[7]   COLONY BANK CONTAINING SYNTHETIC COL EL HYBRID PLASMIDS REPRESENTATIVE OF ENTIRE ESCHERICHIA-COLI GENOME [J].
CLARKE, L ;
CARBON, J .
CELL, 1976, 9 (01) :91-99
[8]   PEMBL - A NEW FAMILY OF SINGLE STRANDED PLASMIDS [J].
DENTE, L ;
CESARENI, G ;
CORTESE, R .
NUCLEIC ACIDS RESEARCH, 1983, 11 (06) :1645-1655
[9]  
HERMAN GE, 1982, J BIOL CHEM, V257, P2605
[10]   INVITRO REPLICATION OF A DAM METHYLATED AND NON-METHYLATED ORI-C PLASMID [J].
HUGHES, P ;
SQUALIHOUSSAINI, FZ ;
FORTERRE, P ;
KOHIYAMA, M .
JOURNAL OF MOLECULAR BIOLOGY, 1984, 176 (01) :155-159