A NONTRADITIONAL ROLE FOR WATER IN THE CYTOCHROME-C-OXIDASE REACTION

被引：97

作者：

KORNBLATT, JA ^{[1
]}

HOA, GHB ^{[1
]}

机构：

[1] INST BIOL PHYS CHIM,INSERM,UNITE 310,F-75005 PARIS,FRANCE

来源：

BIOCHEMISTRY | 1990年 / 29卷 / 40期

关键词：

D O I：

10.1021/bi00492a010

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The passage of electrons through cytochrome c oxidase is directly related to the activity of water. Reducing the activity in a system containing reductant, oxygen, and cytochrome oxidase blocks electron transfer between reduced cytochrome a and oxidized cytochrome a3. The extent of the block is directly related to the osmotic pressure of the system, implying that the protein shell of the oxidase acts as a semipermeable membrane that excludes osmotic perturbants but not water. It appears that approximately 10 water molecules must enter and leave the oxidase in order for internal electron transfer to occur. © 1990, American Chemical Society. All rights reserved.

引用

页码：9370 / 9376

页数：7

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