CORONIN INVOLVED IN PHAGOCYTOSIS - DYNAMICS OF PARTICLE-INDUCED RELOCALIZATION VISUALIZED BY A GREEN FLUORESCENT PROTEIN TAG

Coronin is a protein involved in cell locomotion and cytokinesis of Dictyostelium discoideum. Here we show that coronin is strongly enriched in phagocytic cups formed in response to particle attachment. A fusion of coronin with green fluorescent protein (GFP) accumulates in the cups within less than 1 min upon attachment of a particle and is gradually released from the phagosome within 1 min after engulfment is completed, Phagocytic cup formation competes with leading edge formation and can be interrupted at any stage, When the cup regresses, coronin dissociates from the site of accumulation, TRITC-labeled yeast cells have been used to assay phagocytosis quantitatively in wild-type and coronin-null cells, In the mutant, the rate of uptake is reduced to about one third, which shows that coronin contributes to the efficiency of phagocytosis to about the same extent as it improves the speed of cell locomotion.