THE MSP1-BETA MULTIGENE FAMILY OF ANAPLASMA-MARGINALE - NUCLEOTIDE-SEQUENCE ANALYSIS OF AN EXPRESSED COPY

被引:61
作者
BARBET, AF
ALLRED, DR
机构
关键词
D O I
10.1128/IAI.59.3.971-976.1991
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
A gene for the beta subunit of the immunoprotective surface antigen MSP-1 of Anaplasma marginale was previously cloned and expressed in Escherichia coli. A nucleic acid probe based on this gene detects A. marginale infection in carrier cattle and in the tick vector. We report here the sequence and structural features of the cloned msp1-beta gene and expressed polypeptide. The gene codes for a polypeptide of 756 amino acids that contains domains of tandemly repeated sequence and glutamine-rich regions at the N and C termini. The cloned copy is a member of a multigene family with multiple restriction fragment length polymorphisms in isolates of this rickettsia from different geographical regions. The availability of the sequence will allow use of the polymerase chain reaction in diagnostic assays and the preparation and testing of different vaccine constructs in cattle.
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页码:971 / 976
页数:6
相关论文
共 29 条
[1]   MOLECULAR-BASIS FOR SURFACE-ANTIGEN SIZE POLYMORPHISMS AND CONSERVATION OF A NEUTRALIZATION-SENSITIVE EPITOPE IN ANAPLASMA-MARGINALE [J].
ALLRED, DR ;
MCGUIRE, TC ;
PALMER, GH ;
LEIB, SR ;
HARKINS, TM ;
MCELWAIN, TF ;
BARBET, AF .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1990, 87 (08) :3220-3224
[2]  
AMBROSIO RE, 1986, ONDERSTEPOORT J VET, V53, P179
[3]   CHARACTERIZATION OF AN IMMUNOPROTECTIVE PROTEIN COMPLEX OF ANAPLASMA-MARGINALE BY CLONING AND EXPRESSION OF THE GENE CODING FOR POLYPEPTIDE AM105L [J].
BARBET, AF ;
PALMER, GH ;
MYLER, PJ ;
MCGUIRE, TC .
INFECTION AND IMMUNITY, 1987, 55 (10) :2428-2435
[4]  
BARR PJ, 1986, BIOTECHNIQUES, V4, P428
[5]   A COMPUTER ALGORITHM FOR TESTING POTENTIAL PROKARYOTIC TERMINATORS [J].
BRENDEL, V ;
TRIFONOV, EN .
NUCLEIC ACIDS RESEARCH, 1984, 12 (10) :4411-4427
[6]   ANALYSIS OF SP1 INVIVO REVEALS MULTIPLE TRANSCRIPTIONAL DOMAINS, INCLUDING A NOVEL GLUTAMINE-RICH ACTIVATION MOTIF [J].
COUREY, AJ ;
TJIAN, R .
CELL, 1988, 55 (05) :887-898
[7]  
DENNIS RA, 1970, J AM VET MED ASSOC, V156, P1861
[8]   MOLECULAR-BIOLOGY OF TRYPANOSOME ANTIGENIC VARIATION [J].
DONELSON, JE ;
RICEFICHT, AC .
MICROBIOLOGICAL REVIEWS, 1985, 49 (02) :107-125
[9]   DETECTION AND QUANTITATION OF ANAPLASMA-MARGINALE IN CARRIER CATTLE BY USING A NUCLEIC-ACID PROBE [J].
ERIKS, IS ;
PALMER, GH ;
MCGUIRE, TC ;
ALLRED, DR ;
BARBET, AF .
JOURNAL OF CLINICAL MICROBIOLOGY, 1989, 27 (02) :279-284
[10]   ANALYSIS OF ACCURACY AND IMPLICATIONS OF SIMPLE METHODS FOR PREDICTING SECONDARY STRUCTURE OF GLOBULAR PROTEINS [J].
GARNIER, J ;
OSGUTHORPE, DJ ;
ROBSON, B .
JOURNAL OF MOLECULAR BIOLOGY, 1978, 120 (01) :97-120