The experiment was conducted to study the effect of extruded vs. unprocessed barley in the diet (400 g kg(-1)) on the response of broiler chickens to Trichoderma viride enzyme supplement (cellulase, 10 500 U g(-1); endo-beta-(1:3)(1:4)-glucanase; 24 000 U g(-1) and xylanase, 32 000 U g(-1)). The four experimental diets were as follows: B, unprocessed barley; BE, unprocessed barley + enzyme (200 mg kg(-1)); ExB, extruded barley; ExBE, extruded barley + enzyme (200 mg kg(-1)). The diets were fed as pellets. Extruded compared with unextruded barley gave higher soluble fibre (28 vs. 36 g kg(-1)) and lower insoluble (190 vs. 163 g kg(-1)) and total (219 vs. 200 g kg(-1)) dietary fibre values. Extrusion increased extract viscosity (1.3 vs. 3.7 cP) and water binding capacity (1.6 vs. 2.2 ml g(-1) dry matter) of the barley. During the first week, birds fed extruded barley in the diet increased water consumption (P less than or equal to 0.05), A significant depression in feed efficiency (days 7-21), feed AME, and in fat and protein utilisation was observed when extruded barley was included in the diet. Extrusion reduced the AME content of barley by 0.82 MJ kg(-1) DM. Inclusion of extruded barley in the diet potentiated the weight gain (2.8 vs. 7.6%; P = 0.31) and feed efficiency ( + 0.8 vs. - 6%; P = 0.07) response to dietary enzyme. Greater enzyme effects with the diet containing extruded barley were also found for feed AME (1.6 vs. 2.9%; P = 0.39), fat utilisation (0.4 vs, 5.4%; P less than or equal to 0.05), nitrogen utilisation (-0.5 vs. 5.6%; P = 0.12), as well as beta-glucan degradability (10.4 vs. 15.6%; P = 0.41). In general, the enzyme had more impact on chick performance than barley form.