IDENTIFICATION OF MYELIN-ASSOCIATED GLYCOPROTEIN AS A MAJOR MYELIN-DERIVED INHIBITOR OF NEURITE GROWTH

被引：963

作者：

MCKERRACHER, L ^{[1
]}

DAVID, S ^{[1
]}

JACKSON, DL ^{[1
]}

KOTTIS, V ^{[1
]}

DUNN, RJ ^{[1
]}

BRAUN, PE ^{[1
]}

机构：

[1] MCGILL UNIV, DEPT BIOCHEM, MONTREAL H3G 1Y6, PQ, CANADA

来源：

NEURON | 1994年 / 13卷 / 04期

关键词：

D O I：

10.1016/0896-6273(94)90247-X

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

Contact-dependent axon growth inhibitory activity is present in CNS myelin, but the inhibitory proteins have not been fully characterized. We report here that at least two peaks of inhibitory activity can be separated by fractionating solubilized CNS myelin proteins by DEAE chromatography. A major peak of inhibitory activity corresponded to the elution profile of myelin-associated glycoprotein (MAC). Immunodepletion of MAG from these inhibitory fractions removed neurite growth inhibition, whereas recombinant MAC (ectodomain) was a potent inhibitor of neurite outgrowth. Immunodepletion of MAG from total extracts of CNS myelin restored neurite growth up to 63% of control levels. These results establish that MAC is a significant, and possibly the major, inhibitor in CNS myelin; this has broad implications for axonal regeneration in the injured mammalian CNS.

引用

页码：805 / 811

页数：7

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