THE RELATIONSHIP BETWEEN THE IN-SITU REDUCTION LEVEL OF THE CYTOCHROME-C POOL OF AZORHIZOBIUM-CAULINODANS GROWING IN A CHEMOSTAT WITH NH4+ OR N-2 AS THE N-SOURCE AND THE TOTAL ACTIVITY OF CYTOCHROME-C OXIDASES

被引：0

作者：

PRONK, AF ^{[1
]}

AFFOURTIT, C ^{[1
]}

MASHINGAIDZE, C ^{[1
]}

STOUTHAMER, AH ^{[1
]}

VANVERSEVELD, HW ^{[1
]}

BOOGERD, FC ^{[1
]}

机构：

[1] VRIJE UNIV AMSTERDAM,BIOCENTRUM AMSTERDAM,INST MOLEC BIOL SCI,DEPT MICROBIOL,1081 HV AMSTERDAM,NETHERLANDS

来源：

FEMS MICROBIOLOGY LETTERS | 1995年 / 129卷 / 2-3期

关键词：

OXYGEN; CYTOCHROME C OXIDASE ACTIVITY; CYTOCHROME C; CYTOCHROME REDUCTION LEVEL; CHEMOSTAT; AZORHIZOBIUM CAULINODANS;

D O I：

暂无

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The in situ method for determination of reduction levels of cytochromes b and c pools during steady-state growth (Pronk et al., Anal. Biochem. 214, 149-155, 1993) was applied to chemostat cultures of the wild-type, a cytochrome aa(3) single mutant and a cytochrome aa(3)/d double mutant of Azorhizobium caulinodans. For growth with NH4+ as the N source, the results indicate that (i) the aa(3) mutant strains growing at a dissolved O-2 tension of 0.5% possess an active alternative cytochrome c oxidase, which is hardly present during fully aerobic growth, and assuming that (i) also pertains to the wild-type, (ii) the wild-type uses cytochrome aa(3) under fully aerobic conditions. For growth with N-2 as the N source, it was found that the aa(3) mutant strains growing at dissolved O-2 tensions ranging from 0.5 to 3.0% also contain an active alternative cytochrome c oxidase.

引用

页码：149 / 155

页数：7

共 19 条

[1] ELECTRON TRANSPORT SYSTEMS OF RHIZOBIUM JAPONICUM .2. RHIZOBIUM HAEMOGLOBIN CYTOCHROMES AND OXIDASES IN FREE-LIVING (CULTURED) CELLS [J].