BOMBYX-MORI SILK FIBROIN-BASED IMMOBILIZATION METHOD FOR PREPARATION OF UREA ELECTRODE

被引：7

作者：

FANG, Y

SHAO, ZZ

DENG, JQ

YU, TY

机构：

[1] FUDAN UNIV,DEPT CHEM,SHANGHAI 200433,PEOPLES R CHINA

[2] FUDAN UNIV,DEPT MAT SCI,SHANGHAI 200433,PEOPLES R CHINA

来源：

ELECTROANALYSIS | 1992年 / 4卷 / 06期

关键词：

UREA; BIOSENSOR; ENZYME IMMOBILIZATION;

D O I：

10.1002/elan.1140040611

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A novel method for enzyme immobilization was developed. This system represents the first successful use of silk fibroin (obtained directly from Living Bombyx mori) as immobilization materials for the construction of an urea electrode. It is based on the structural transition of the silk fibroin membrane from alpha-helix and random coil to antiparallel beta-sheet. Operational conditions and response characteristics of the resulting urea electrode were investigated, including immersion time, pH, buffer, response time, calibration curve, recovery, thermal stability, and lifetime.

引用

页码：669 / 672

页数：4