CHITIN DEACETYLATION BY ENZYMATIC MEANS - MONITORING OF DEACETYLATION PROCESSES

被引：78

作者：

MARTINOU, A

KAFETZOPOULOS, D

BOURIOTIS, V

机构：

[1] INST MOLEC BIOL & BIOTECHNOL, DIV ENZYME TECHNOL, GR-71110 CRETE, GREECE

[2] UNIV CRETE, DEPT BIOL, DIV APPL BIOL & BIOTECHNOL, GR-71110 CRETE, GREECE

来源：

CARBOHYDRATE RESEARCH | 1995年 / 273卷 / 02期

关键词：

CHITIN; CHITOSAN; CHITIN DEACETYLASE; MUCOR ROUXII; CHITIN DEACETYLATION;

D O I：

10.1016/0008-6215(95)00111-6

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A method for monitoring enzymatic deacetylation processes of natural or artificial chitin substrates as well as N-acetylchitooligosaccharides by the direct determination of the acetate released is described. Furthermore, a new assay is presented for the determination of chitin deacetylase activity employing hexa-N-acetylchitohexaose [(GlcNAc)(6)] as substrate and measuring the acetate released enzymatically. The K-m value for (GlcNAc)(6) has been determined as well as the pH and temperature dependence of activity and the thermostability of the enzyme. Finally, initial studies on the effectiveness of the enzyme on various chitin and chitosan substrates are presented.

引用

页码：235 / 242

页数：8

共 22 条

[1]

[Anonymous], 1975, MAKROMOL CHEM, DOI DOI 10.1002/MACP.1975.021760426

[2] PATHWAY OF CHITOSAN FORMATION IN MUCOR-ROUXII - ENZYMATIC DEACETYLATION OF CHITIN [J].