STUDIES ON HUMAN PORIN .5. THE EXPRESSION OF PORIN-31HL IN THE PLASMALEMM IS NOT CAUSED BY CELL-TRANSFORMATION

被引：33
作者：
KONIG, U
GOTZ, H
WALTER, G
BABEL, D
HOHMEIER, HE
THINNES, FP
HILSCHMANN, N
机构：
[1] MAX PLANCK INST EXPTL MED,IMMUNOCHEM ABT,HERMANN REIN STR 3,W-3400 GOTTINGEN,GERMANY
[2] BEHRINGWERKE AG,W-3550 MARBURG 1,GERMANY
来源：
BIOLOGICAL CHEMISTRY HOPPE-SEYLER | 1991年 / 372卷 / 08期
关键词：
PORIN; VDAC; PLASMA MEMBRANE; TRANSFORMED CELLS; NORMAL LYMPHOCYTES; EPITHELIAL CELLS; CYSTIC FIBROSIS;
D O I：
10.1515/bchm3.1991.372.2.565
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
In recent papers we proved "Porin 31HL" to be located on the surface of human, EBV-transformed B lymphocytes. Here we present proof of "Porin 31HL" in the plasmalemm of normal human blood lymphocytes. For this purpose B and Tlymphocytes were isolated from human heparinized blood and examined by indirect immunofluorescence techniques using different monoclonal antibodies against purified "Porin 31HL" and some B and T cell markers, respectively. For comparison a number of established cell lines of different origin were employed. Hence it followed that normal B and T cells as well as transformed and leukemic cells express "Porin 31HL" in their membrane. No significant quantitative differences could be seen. Consequently, the location of "Porin 31HL" in the plasmalemm is not a product of transformation.
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页码：565 / 572
页数：8
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