GLYCOSYLATION AND FUNCTIONAL-ACTIVITY OF ANTI-D SECRETED BY 2 HUMAN LYMPHOBLASTOID CELL-LINES

被引：3
作者：
CANT, D
BARFORD, J
HARBOUR, C
FLETCHER, A
PACKER, N
GOOLEY, A
机构：
[1] UNIV SYDNEY, DEPT INFECT DIS, SYDNEY, NSW 2006, AUSTRALIA
[2] NSW RED CROSS BLOOD TRANSFUS SERV, SYDNEY, NSW 2000, AUSTRALIA
[3] MACQUARIE UNIV, CTR ANALYT BIOTECHNOL, N RYDE, NSW 2113, AUSTRALIA
来源：
CYTOTECHNOLOGY | 1994年 / 15卷 / 1-3期
关键词：
ANTI-D; EFFECTOR FUNCTION; GLYCOSYLATION; IMMUNOGLOBULIN G; MONOCLONAL ANTIBODY;
D O I：
10.1007/BF00762397
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
Cell lines BTSN4 and BTSN5 were produced by the Epstein-Barr Virus (EBV) transformation of B-lymphocytes from the same human donor. Both secrete an anti-D monoclonal of the IgG1 subclass but these antibodies display vastly different effector activities. Specifically, anti-D from BTSN4 has a far greater activity in both monocyte and lymphocyte-mediated ADCC reactions and causes a higher percentage of rosettes to be formed with monocyte-like U937 cells. This variation in functional activity is shown to coincide with changes in the structure of the sugar chains attached to the asparagine-297 site on the immunoglobulin heavy chain.
引用
收藏
页码：223 / 228
页数：6
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