STRUCTURAL-ANALYSIS OF PKS1, A POLYKETIDE SYNTHASE GENE INVOLVED IN MELANIN BIOSYNTHESIS IN COLLETOTRICHUM-LAGENARIUM

被引：190

作者：

TAKANO, Y ^{[1
]}

KUBO, Y ^{[1
]}

SHIMIZU, K ^{[1
]}

MISE, K ^{[1
]}

OKUNO, T ^{[1
]}

FURUSAWA, I ^{[1
]}

机构：

[1] KYOTO UNIV,FAC AGR,PLANT PATHOL LAB,KYOTO 60601,JAPAN

来源：

MOLECULAR & GENERAL GENETICS | 1995年 / 249卷 / 02期

关键词：

PLANT PATHOGENIC FUNGUS; COLLETOTRICHUM LAGENARIUM; MELANIN; ALBINO MUTANT; POLYKETIDE SYNTHASE;

D O I：

10.1007/BF00290362

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Albino mutants (Pks(-)) of Colletotrichum lagenarium form nonmelanized appressoria and possess little penetrating ability on the host plant. The defect in albino mutant 79215 (Pks(-)) is considered to lie in pentaketide biosynthesis and/or pentaketide cyclization during melanin biosynthesis. The cosmid pAC7, carrying the PKS1 gene, when transformed into the albino mutant restores the wild-type melanin phenotype. We have determine the DNA sequence and the transcriptional organization of the PKS1 gene. The PKS1 gene contains one open reading frame, consisting of 3 exons separated by two short introns. The predicted PKS1 polypeptide consists of 2187 amino acids and shows significant similarities with other polyketide synthases, particularly that encoded by wA in Aspergillus nidulans, involved in conidial pigmentation. The PKS1 gene contains highly conserved beta-ketoacyl synthase, acetyl/malonyl transferase, and acyl carrier protein domains. We propose that the C. lagenarium PKS1 gene encodes a polyketide synthase involved in melanin biosynthesis.

引用

页码：162 / 167

页数：6

共 23 条

[1] THE MULTIFUNCTIONAL 6-METHYLSALICYLIC ACID SYNTHASE GENE OF PENICILLIUM-PATULUM - ITS GENE STRUCTURE RELATIVE TO THAT OF OTHER POLYKETIDE SYNTHASES [J].