AUTOMATED FLOW-INJECTION SPECTROPHOTOMETRIC DETERMINATION OF PARA-SUBSTITUTED AND META-SUBSTITUTED PHENOLS OF PHARMACEUTICAL INTEREST BASED ON THEIR OXIDATIVE CONDENSATION WITH 1-NITROSO-2-NAPHTHOL

The reaction of para- and meta-substituted phenols with 1-nitroso-2-naphthol in the presence of either CeIV or Pb IV as an oxidant has been used to develop a fast automated flow injection (Fl) method. A stopped-flow kinetic study of the reaction revealed the optimum conditions for the proposed Fl method. Acetaminophen, amoxicillin, cefadroxil, isoxsuprine, nylidrin, propylparaben, tyrosine and metaraminol can be determined in the range 1 × 10-4-8 × 10-4 M, with relative standard deviations of <2%, and a measurement throughput of 40 measurements h-1. The method was evaluated by performing interference studies of common excipients and assaying commercial formulations of acetaminophen and isoxsuprine. The results were in good agreement with those obtained by acceptable spectrophotometric or high-performance liquid chromatographic methods (mean difference 2.1%). The high sample throughput of the Fl method was exploited by performing a content uniformity test of isoxsuprine tablets.